Virus:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
Recombinant:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
Saline:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
Staining:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
dsDNA Assay:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
Amplification:Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains C57BL/6J The Jackson Laboratory JAX stock #000664 RRID:IMSR_JAX:000664 Iga / (Ighatm1Grh) SPF Vivarium LASC Schlieren (Harriman et al., 1999) MGI: 1857185 Recombinant DNA NICE pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) MoBiTec GmbH Cat# VS-ELV00750-01 Software and algorithms FlowJo v10.7 https://www.flowjo.com/ RRID:SCR_008520 FACSDiva 9.1.2 BD Bioscience RRID:SCR_001456 GraphPad Prism 10.1.0 https://www.graphpad.com RRID:SCR_002798 NDP.view2 Viewing software U12388-01 Hamamatsu Photonics N/A FastQC 0.11.8 RRID:SCR_014583 OPEN ACCESS
Article Title: Protection from environmental enteric dysfunction and growth improvement in malnourished newborns by amplification of secretory IgA
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-mouse IgM alkaline phosphatase (AP) conjugated Southern Biotech Cat.#:1021-04; RRID:AB_2794239 Mouse IgA unlabeled Southern Biotech Cat.#:0106-01; RRID:AB_2714214 Anti-IgA biotin conjugated Southern Biotech Cat.#:1040-08; RRID:AB_2794374 Bacterial and virus strains L.lactisPNZ In this paper N/A L.lactisPNZ-Apyr In this paper N/A Chemicals, peptides, and recombinant proteins Streptavidin PercP/Cy5.5 BioLegend Cat.#:405213 Streptavidin eF450 eBioscience Cat.#:48-4317-82 Streptavidin horseradish peroxidase conjugate (HRP) Invitrogen Cat.#:S911 FITC-Dextran (MW4000) Sigma Aldrich Cat.#:D8537 HEPES Gibco Cat.#:15630-056 M17 medium Merk Cat.#:CM0817B Glucose Sigma Aldrich Cat.#:G7021 Nisin Sigma Aldrich Cat.#:N5764 Schaedler agar Sigma Aldrich Cat.#:91019 Bovine Serum Albumine Sigma Aldrich Cat.#:A7906 RPMI Medium 1640(1x) Gibco Cat.#:42401-018 Penicillin/streptomycin Gibco Cat.#:15070-063 Kanamycin Gibco Cat.#:15160-047 EDTA - solution pH 8,0 Axon Lab Cat.#:A3145.1000 0.05% Trypsin-EDTA Gibco Cat.#.25300-054 Collagenase D from Clostridium histolyticum Sigma Aldrich Cat.#:11088882001 DNAse I grade II Sigma Aldrich Cat.#:10104159001 Fetal bovine serum Gibco Cat.#:10270-106 Percoll Cytiva Cat.#:17089101 Dulbecco’s Phosphate Buffered Saline (PBS) Sigma-Aldrich Cat.#:D8537 SYTOTM BC Green Fluorescent Nucleic Acid Stain Life Technologies Cat.#:S34855 Goat serum Sigma Cat.#:G9023 Parafolmadehyde Merk Cat.#:30525-89-4 Cholera Toxin antigen Sigma-Aldrich Cat.#:C8052 4-Nitrophenyl Phosphate disodium sal hexahydrate Sigma-Aldrich Cat.#:N2765-100TAB Na2CO3/L Merk Cat.#:1.06395.0500 NaCO3/L Applichem Cat.#:A0384.0500 Souybean trypsin inhibitor Sigma-Aldrich Cat.#:T6522 Phenylmethanesylfonyl fluoride (PMSF) Sigma-Aldrich Cat.#:P7625 Cholera toxin (CT) Sigma-Aldrich Cat.#:C8052 Chicken ovalbumin Sigma-Aldrich Cat.#:A5503 Critical commercial assays Comassie PlusTM Protein assay Thermo Fisher Cat.#:23236 DuoSet ELISA Mouse Lipocalin-2/NGAL R&D Systems Cat.#:DY1857 ATP Determination Kit Invitrogen Cat.#:A22066 Fast DNA Stool Mini Kit Quiagen Cat.#51604 Fixation/Permeabilization Solution Kit BD Bioscience Cat.#557414 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat.#00-5523-00 Quant-iTTM PicoGreenTM dsDNA Assay Kits Thermo Fisher Cat.#P7589 Mouse Lipocalin-2/NGAL DuoSet ELISA R&D Systems Cat.#:DY1857 Deposited data 16S rRNA amplicon sequences In this paper BioProject: PRJEB68326 (Continued on next page) Cell Reports Medicine 5, 101639, July 16, 2024 e2 Article ll OPEN ACCESS ..
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